The method (3D-morphometry, ComServ, Austria) for dimensional and angular measurements of microstructures imaged in the SEM was developed in our lab in 1999. It uses digitized stereopaired images frame-grabbed (slow scan) directly from the SEM’s photo-display, vector equation-based algorithms for calculation of spatial co-ordinates and derived distance- and angular measurements, and offers dynamic data exchange (DDE) together with on-line graphs of frequency distributions of measured variables. Formulas for central perspective depth computation allow the overall error to be less than 1.0%.
The method was further improved in 2008 and the new java based software is now capable of using it with standard operating systems. A modern graphical user interface (GUI) and new data exchange and presentation capabilities are supported. The improved usability facilitates easy to use and understand measurement processes. This technique is currently used to analyze
a) the geometry of microvascular trees and
b) to determine bifurcation indices and area ratios followed by a calculation of optimality principles which are realized within single arterial bifurcations respectively venous mergings.
These unique features combined with an easy-to-use Windows interface provides you with effortless analyses. M3 can handle all your 3D image analyzing tasks quickly and accurately. The use of data exchange allows you to fully explore and document your results within Open Office.
Detailed information about the method underlying the morphometric 3D measurements and vascular optimality calculations of M3 can be obtained from the following original book chapter: